The MPX Blotting System is ideal for any multiple-target Western blot application. This system increases Western blot throughput, maximizes efficiency, and reduces relative overall cost.
Screen multiple samples and multiple targets all on the same blot without cutting, stripping, or cross-contamination.
Use the MPX Blotter for a variety of assays, such as primary and secondary antibody screening, monoclonal antibody screening, blocking buffer optimization as well as multi-target probing of 1 - 4 samples on a single membrane. Most common Western procedures that generate a PVDF or nitrocellulose blot of 7 cm x 8.5 cm can be adapted to the MPX format.
Channel ports are conveniently spaced, staggered, and beveled to create a simple workflow for using both standard and multi-channel pipettes. The low volume channel ports accommodate a maximum volume of 160 µL, thus conserving precious and costly antibody. The nesting dovetail joint design allows multiple MPX units to be linked together for easy-to-handle multiple-blot processing.
Processing is as simple as clamping the blot into the MPX, creating up to 24 independent channels. The range of usable channels per sample is relative to comb-size – from 24 on a single sample to four on a four-sample blot.
Using LI-COR’s selection of IRDye® dye-conjugated secondary antibodies on the Odyssey Imagers provides a maximum of 48-targets on a single membrane— two per channel—and the option for quantitative analysis. Other detection methods, such as standard chemiluminescence and colorimetric, can provide a maximum 24 targets.
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