α-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of α-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, α-tubulin primary antibodies can be used for the detection of α-tubulin when performing two-color detection.
Detect α-Tubulin Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
α-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.Do not aliquot the antibody.
| Properties | α-Tubulin Mouse Monoclonal Antibody (P/N 926-42213) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 52 kDa |
| Isotype | Mouse IgG1 |
| Specificity/Sensitivity | Detects endogenous levels of total α-tubulin protein. α/β-tubulin heterodimers form the tubulin subunit common to all eukaryotic cells. |
| Immunogen | Full-length chicken α-tubulin (purified from brain extracts) |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42213: RRID AB_2637092
Additional information
Selected P/N: 926-42213
Price
$365.93
Powered by BiozWhen using an HKP as an ILC for normalization, it’s important to validate the HKP for each experiment to ensure its expression is stable.
Many factors can influence expression including tissue, treatment, and cell density.