β-Actin Mouse Monoclonal Antibody for Normalization
The β-actin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-actin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-actin primary antibodies can be used for the detection of β-actin when performing two-color detection.
Detect β-Actin Mouse Primary Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
β-Actin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | β-Actin Mouse Monoclonal Antibody (P/N 926-42212) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey, hamster |
| Target Molecular Weight | 45 kDa |
| Isotype | Mouse IgG2b |
| Specificity/Sensitivity | Detects endogenous levels of β-actin protein |
| Immunogen | A synthetic peptide (KLH-coupled) that corresponds to the residues near the amino-terminus of human β-actin |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42212: RRID AB_2756372
Actin Used for Normalization
Additional information
Powered by BiozWhen using an HKP as an ILC for normalization, it’s important to validate the HKP for each experiment to ensure its expression is stable.
Many factors can influence expression including tissue, treatment, and cell density.
