The β-Tubulin primary antibody can be used as an internal loading control (ILC) for normalization.
The expression of β-tubulin, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, β-tubulin primary antibodies can be used for the detection of β-tubulin when performing two-color detection.
Detect β-Tubulin Rabbit Polyclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
β-Tubulin antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | β-Tubulin Rabbit Polyclonal Antibody (P/N 926-42211) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey, bovine |
| Target Molecular Weight | 55 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total β-tubulin protein. Does not cross-react with recombinant α-tubulin. |
| Immunogen | A synthetic peptide (KLH-coupled) that corresponds to the sequence of total β-tubulin, and does not cross-react with recombinant α-tubulin |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F) |
RRID
- P/N 926-42211: RRID AB_1850029
β-Tubulin Detected in Various Cell Lines
Additional information
Selected P/N: 926-42211
Price
$365.93
Powered by BiozWhen using an HKP as an ILC for normalization, it’s important to validate the HKP for each experiment to ensure its expression is stable.
Many factors can influence expression including tissue, treatment, and cell density.