Intercept® (TBS) Blocking Buffer

Intercept (TBS) Blocking Buffers are formulated to provide highly efficient blocking and low background variability for replicable quantitative Western blots and other immunoassays. Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in Tris-buffered saline (TBS).

• Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies.
• Can be used with the widest range of targets for Western blot detection.
• Ideal for primary antibodies that are optimized for Tris-based buffer systems.
• Can be used for detection of phosphorylated targets.
• Has superior performance for alkaline phosphatase detection.
• Excellent choice for biotin-streptavidin detection.

For larger pack sizes, please contact your local sales office.

Formulation

Intercept (TBS) Blocking Buffer is a ready-to-use blocker formulation in TBS. It contains no sodium azide, making it suitable for both near-infrared or chemiluminescent detection. It is stored at 4 °C and is also available in kits with Immobilon^®-FL PVDF and nitrocellulose membranes.

Shake well before each use.

Intercept (TBS) Blocking Buffer can be used for many immunoassays and applications, including:

• Quantitative Western Blot
• Chemiluminescent Western Blots
• In-Cell Western™ Assay
• In-Gel Western
• Protein Array
• Glycoprotein Detection