Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a constitutively expressed housekeeping protein (HKP). The GAPDH primary antibody can be used as an internal loading control for normalization.
The expression of GAPDH, or any HKP, should be validated to ensure that its expression does not change under experimental conditions.
Once validated, GAPDH primary antibodies can be used for the detection of GAPDH when performing multiplex Western blot detection.
Detect GAPDH Rabbit Monoclonal Antibody with IRDye® Goat anti-Rabbit or IRDye Donkey anti-Rabbit secondary antibodies.
Other options for housekeeping protein normalization
Reactivity and Specificity
GAPDH antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | GAPDH Rabbit Monoclonal Antibody (P/N 926-42216) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 37 kDa |
| Isotype | Rabbit IgG |
| Specificity/Sensitivity | Detects endogenous levels of total GAPDH protein. May cross-react with pig |
| Immunogen | A synthetic peptide that corresponds to residues near the carboxy terminus of human GAPDH |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) |
RRID
- P/N 926-42216: RRID AB_2814901
GAPDH Rabbit Monoclonal Antibody in HeLa, NIH/3T3, and COS-7 Lysates
Additional information
Selected P/N: 926-42216
Price
$365.93
Powered by BiozWhen using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.
Many factors can influence expression including tissue, treatment, and cell density.