CellTag 520 Stain is a visible fluorescent, non-specific cell stain that provides accurate normalization to cell number for In-Cell Western™ Assay applications.
The stain accumulates in both the nucleus and cytoplasm of permeabilized cells, and provides linear fluorescent signal across a wide range of cell types and cell numbers.
CellTag 520 Stain is detected in the 520 nm channel of the LICORbio Atlas™ Imager, as well as Odyssey® M Imagers and Odyssey F Imagers. CellTag 520 Stain is applied to the cells during incubation with an IRDye® Secondary Antibody (e.g., IRDye 680RD or IRDye 800CW) and enables accurate measurement of target protein levels with much higher-throughput than Western blotting.
Properties
- Size: 2 x 50 nmole (lyophilized)
- λEx/λEm: 548/563 nm
- Recommended concentration: 0.1 μM
Linear Relationship Between Fluorescence and Cell Number
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