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IRDye 680LT Maleimide will label molecules with free sulfhydryl (–SH) groups, such as cysteine residues in proteins. Conjugation can be performed at physiological pH.
Which 700 Channel Dye Should I Use?Note: IRDye 680LT dye products should not be used for small animal in vivo imaging or In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
Dye Structure
Properties
- Chemical Formula: C56H55N5Na5O21S6
- Molecular Weight: 1427.37 g/mol
- Exact Mass: 1426.12
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 250,000 | 680 nm | 694 nm |
| 1X PBS | 250,000 | 676 nm | 693 nm |
Absorption and Emission Spectra in 1X PBS
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