Assays that use IRDye 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.
Note: The carboxylate dye has no reactive group and cannot be used for labeling.
As a control for cell-based assays that monitor binding of a dye-labeled agent.
For evaluation of behavior and clearance of the dye itself.
As a standard to determine the amount of unreacted ("free") dye after IRDye 680RD conjugation and purification.
Residual unreacted dye may cause:
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