IRDye® 680RD Protein Labeling Kits

IRDye 680RD Protein Labeling Kits can be used to label antibodies and other proteins for Western blotting, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section applications.

High Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 45 - 200 kDa.

This kit contains:

• 3 x 0.1 mg IRDye 680RD Reactive Dye vials
• 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
• 25 mL 1X PBS
• 0.5 mL ultra-pure water
• 3 x Pierce^® Zeba™ Desalting Spin Columns (P/N 89891)
Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
• Pierce Zeba Desalting Spin column instructions
• Protocol for IRDye 680RD Protein Labeling Kit - High MW

Low Molecular Weight

Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.

This kit contains:

• 3 x 0.5 mg IRDye 680RD Reactive Dye vials
• 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
• 25 mL 1X PBS
• 0.5 mL ultra-pure water
• 3 x Pierce Zeba Desalting Spin Columns (P/N 89891)
Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
• Pierce Zeba Desalting Spin column instructions
• Protocol for IRDye 680RD Protein Labeling Kit - Low MW

Microscale

Optimized for labeling 100 μg of protein with molecular weight 14 - 200 kDa.

This kit contains:

• 3 x 0.1 mg IRDye 680RD Reactive Dye vials
• 0.5 mL 1 M Potassium Phosphate (K₂HPO₄), pH 9
• 25 mL 1X PBS
• 0.5 mL ultra-pure water
• 3 x Pierce Zeba Desalting Spin Columns (P/N 89892)
Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
• Pierce Zeba Desalting Spin Column instructions
• Protocol for IRDye 680RD Protein Labeling Kit - Microscale