IRDye® 800CW Azide Infrared Dye

IRDye 800CW Azide provides the functionality for preferential labeling of molecules that contain either the alkyne or dibenzocyclooctyne (DBCO) group.

Learn more about Near-Infrared Fluorescent Click Chemistry Reagents.

Dye Structure

Properties

  • Chemical Formula: C54H67N6Na3O17S4
  • Molecular Weight: 1269.36 g/mol
  • Exact Mass: 1268.31
  • Correction factor at 260 – 280 nm: 3%

Absorption and Emission Spectra

Solvent Absorption Maxima Emission Maxima
Methanol 778 nm 794 nm
1X PBS 774 nm 789 nm
1:1 Methanol to 1X PBS 778 nm

Absorbance and Emission Spectra in 1X PBS

Molar Absorptivity

Solvent Molar Absorptivity (L mol-1 cm-1)
Methanol 300,000
1X PBS 240,000
1:1 Methanol to 1X PBS 270,000

Example Data

Palmitoylation of Lck kinase analyzed with a Click Chemistry IRDye reagent.​​ Lck was immunoprecipitated from metabolically-labeled Jurkat cell lysates. Palmitoylation was detected with CuAAC click chemistry and IRDye 800CW Azide Infrared Dye​ (green signals; P/N 929-60000). Total Lck was detected with anti-Lck D88 antibody and IRDye 680RD Donkey anti-Rabbit IgG​ (red; P/N 926-68073). Yellow indicates overlap of signals. The mobility shift caused by palmitoylation is observed in lanes 3-5 and 7-9 (green bands migrating more slowly than red bands). Data and image supplied by a LI-COR customer.
Selected P/N: 929-60000
Price
$397.70
SDSPack Insert

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