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IRDye 800CW Maleimide provides the functionality for labeling molecules that contain free sulfhydryl (–SH) groups, such as cysteine residues in proteins, with IRDye 800CW. The reactive group of the IRDye 800CW Maleimide allows conjugation reactions to be performed very efficiently at physiological pH.
Dye Structure
Properties
- Chemical Formula: C52H57N4Na3O16S4
- Molecular Weight: 1191.25 g/mol
- Exact Mass: 1190.23
Absorption and Emission Spectra
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima |
|---|---|---|---|
| Methanol | 300,000 | 778 nm | 794 nm |
| Water | 240,000 | 774 nm | 789 nm |
| 1X PBS | 240,000 | 774 nm | 789 nm |
| PBS: Methanol | 270,000 | 777 nm | 791 nm |
Absorption and Emission Spectra in 1X PBS
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