IRDye 800CW Protein Labeling Kits can be used to label antibodies and other proteins for applications such as Western blots, In-Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays.
High Molecular Weight
Optimized for labeling 1.0 mg amounts of protein with molecular weight 45 - 200 kDa.
This kit contains:
- 3 x 0.1 mg IRDye 800CW Reactive Dye vials
- 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
- 25 mL 1X PBS
- 0.5 mL ultra-pure water
- 3 x Pierce® Zeba™ Desalting Spin Columns (P/N 89891) Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
- Pierce Zeba Desalting Spin column instructions
- Protocol for IRDye 800CW Protein Labeling Kit - High MW
Low Molecular Weight
Optimized for labeling 1.0 mg of protein with molecular weight 14 - 45 kDa.
This kit contains:
- 3 x 0.5 mg IRDye 800CW Reactive Dye vials
- 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
- 25 mL 1X PBS
- 0.5 mL ultra-pure water
- 3 x Pierce Zeba Desalting Spin Columns (P/N 89891) Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
- Pierce Zeba Desalting Spin column instructions
- Protocol for IRDye 800CW Protein Labeling Kit - Low MW
Microscale
Optimized for labeling 100 μg of protein with molecular weight 14 - 200 kDa.
This kit contains:
- 3 x 0.1 mg IRDye 800CW Reactive Dye vials
- 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9
- 25 mL 1X PBS
- 0.5 mL ultra-pure water
- 3 x Pierce Zeba Desalting Spin Columns (P/N 89892) Note: The minimum recommended protein molecular weight for these columns is 7 kDa. See IRDye Peptide Labeling Guide for options.
- Pierce Zeba Desalting Spin Column instructions
- Protocol for IRDye 800CW Protein Labeling Kit - Microscale
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