IRDye® 800CW Carboxylate

Assays that use IRDye 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.

How is IRDye 800CW Carboxylate Used?

In vitro Cell-based Assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

• Validation of optical agents for in vivo administration
• Evaluation of binding specificity

In vivo Imaging

For evaluation of behavior and clearance of the dye itself.

• Timing of dye clearance from the animal's body
• Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.

Residual unreacted dye may cause:

• Artificially high values when dye/protein (D/P) ratio is calculated
• Increased background fluorescence in biological assays

Dye Structure

Properties

• Chemical Formula: C₄₆H₅₀N₂Na₄O₁₅S₄
• Molecular Weight: 1091.10 g/mol
• Exact Mass: 1090.17