IRDye 800RS infrared dye is an excellent choice for nucleic acid applications. This near-infrared fluorescence dye has good water solubility, but low salt tolerance. It is more hydrophobic than IRDye 800CW.
IRDye 800RS NHS ester can be used to label the primary and secondary amino groups of DNA and RNA. Nucleic acids that have been labeled with IRDye 800RS can be easily purified by reverse-phase chromatography.
IRDye Infrared Dyes Overview
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima | Emission Maxima | Stokes Shift |
|---|---|---|---|---|
| Methanol | 300,000 | 770 nm | 786 nm | 16 nm |
| Water | 200,000 | 767 nm | 786 nm | 19 nm |
| 1X PBS | 200,000 | 767 nm | 786 nm | 19 nm |
| PBS: Methanol | 270,000 | 770 nm | 786 nm | 16 nm |
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