IRDye QC-1 is the first non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). It has the widest available quenching range, so you do not need to carefully match the donor's fluorescence spectrum with the acceptor's absorbance. IRDye QC-1 quenches common fluorophores with >97% efficiency1.
For more information, see “Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores” below.
IRDye QC-1 NHS ester can be used for FRET (fluorescence resonance energy transfer) applications such as protease assays. Recently, it has been paired with IRDye 800CW in optoacoustic studies.
IRDye Infrared Dyes OverviewDye Structure
Properties
- Chemical Formula: C53H62C|N4Na3O16S4
- Molecular Weight: 1243.75 g/mol
- Exact Mass: 1242.24
Absorption Spectrum
| Solvent | Ext. Coeff. (M-1cm-1) | Absorption Maxima |
|---|---|---|
| 1X PBS | 96,000 | 737 nm |
| Water | 98,000 | 788 nm |
Absorption Spectrum in 1X PBS
Absorption Spectrum in Methanol
Spectral Overlap of IRDye QC-1 Absorption with Common Donor Fluorophores
Reference
- Peng, X et al. A nonfluorescent, broad range quencher dye for Fõrster resonance energy transfer assays. Anal Biochem. 388(2):220-28 (2009).
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