Special pricing is available in your location. For more information, please contact your local expert here.
Special pricing is available in your location. For more information, please contact your local expert here.
Highly cross-adsorbed donkey (polyclonal) anti-chicken IgG (H+L) antibody conjugated to IRDye 680LT.
Chicken IgY, whole molecule. IgY is the original designation for the IgG-like protein found in both serum and egg yolk.
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads. Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule chicken IgY, and with the light chains of other chicken immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reactivity with bovine, goat, guinea pig, Syrian hamster, horse, human, mouse, rabbit, rat, and sheep serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
Recommended for:
Not Recommended for:
IRDye 680LT Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
Application | Recommended | Suggested Range |
---|---|---|
Odyssey Western blot detection | 1:20,000 | 1:10,000 - 1:50,000 |
Other | User optimized |
Optimum dilutions will vary and should be determined empirically.