Highly cross-adsorbed goat anti-mouse IgG2a (γ 2a chain specific) antibody conjugated to IRDye 800CW.
Mouse IgG2a paraproteins
Isolation of specific antibodies was accomplished by affinity chromatography on mouse IgG2a covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy chain of mouse IgG2a. This antibody has been tested by dot blot and/or solid-phase adsorbed to ensure minimal cross-reactivity with mouse IgM, IgG1, IgG2b, IgG3, and IgA, pooled human sera, and purified human paraproteins. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western™ Assay applications. Results with your primary antibody may vary and specificity should be confirmed prior to performing two-color detection.
Highly recommended for:
IRDye 800CW Secondary Antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
| Application | Recommended | Suggested Range |
|---|---|---|
| Odyssey Western blot detection | 1:20,000 | 1:5,000 - 1:25,000 |
| In-Cell Western Assay detection | 1:800 | 1:200 - 1:1,200 |
| Other | User optimized |
Optimum dilutions will vary and should be determined empirically.
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