The Histone H3 primary antibody can be used as an internal loading control for normalization and is particularly effective when detecting target proteins in nuclear extracts.
The expression of Histone H3, or any housekeeping protein (HKP), should be validated to ensure that its expression does not change under experimental conditions.
Once validated, Histone H3 primary antibodies can be used for the detection of Histone H3 when performing multiplex Western blot detection.
Detect Histone H3 Mouse Monoclonal Antibody with IRDye® Goat anti-Mouse or IRDye Donkey anti-Mouse secondary antibodies.
Other options for housekeeping protein normalization
Histone H3 antibody is supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and <0.02% sodium azide.
Do not aliquot the antibody.
| Properties | Histone H3 Mouse Monoclonal Antibody (P/N 926-42218) |
|---|---|
| Species Cross-Reactivity | Human, mouse, rat, monkey |
| Target Molecular Weight | 17 kDa |
| Isotype | Mouse IgG3 |
| Specificity/Sensitivity | Detects endogenous levels of total Histone H3 protein |
| Immunogen | A peptide that is specific to the carboxy terminus of human histone H3 protein |
| Tested Applications | Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (F), Chromatin Immunoprecipitation (CHIP) |
When using an HKP as your normalization strategy, it’s important to validate the HKP for each experiment to ensure its expression is stable.
Many factors can influence expression including tissue, treatment, and cell density.
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