The Chameleon Duo Pre-stained Protein Ladder provides multi-colored, pre-stained bands for visual inspection and two-color near-infrared detection.
Not sure which protein marker to choose? Simply refer to the table below.
*Chameleon Vue can be detected with chemiluminescence when used with the WesternSure Pen, P/N 926-91000.
Yes. Chameleon Duo Pre-stained Protein Ladder can be used with MOPS-based and other commonly used SDS-PAGE gel and buffer systems. As with other pre-stained protein ladders, apparent migration and band spacing can vary with gel chemistry and running buffer, so molecular-weight positions should be interpreted within the specific electrophoresis system being used.
The Chameleon Duo Pre-stained Protein Ladder is used as a molecular weight reference for protein electrophoresis and Western blotting. Its pre-stained protein bands help researchers monitor protein separation, transfer, and molecular weight during the Western blot workflow.
Yes. The bands in the Chameleon Duo Pre-stained Protein Ladder are conjugated with IRDye 680RD and IRDye 800CW dyes, allowing the ladder to be detected in near-infrared fluorescent Western blot workflows alongside fluorescently detected target proteins.
Yes. Because the ladder contains pre-stained proteins, bands can be visually monitored during electrophoresis and transfer, helping researchers evaluate gel separation and protein transfer before imaging the membrane.
A pre-stained protein ladder provides immediate visual confirmation of electrophoresis and transfer while also providing molecular weight references for protein bands. This can make it easier to identify problems with a Western blot before imaging and analysis.
Yes. The Chameleon Duo Pre-stained Protein Ladder is designed for fluorescent Western blot workflows and can be imaged using compatible Odyssey imagers and the LICORbio Atlas imager.
The Odyssey One-Color Protein Molecular Weight Marker provides fluorescent molecular weight reference bands in the 700 nm channel for Western blot imaging. The Chameleon Duo Pre-stained Protein Ladder provides visible bands for monitoring protein separation and transfer and is also conjugated with IRDye® 680RD and IRDye 800CW dyes for near-infrared detection. Chameleon Duo is a better choice when both visual monitoring and near-infrared detection are desired, while the Odyssey One-Color Marker provides a single-channel fluorescent molecular weight reference for Western blot imaging.
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