Tested for the preparation of staining solutions for polyacrylamide gel electrophoresis, this triphenylmethane dye is used for protein detection in gel electrophoresis and in the Bradford method for determining protein concentration. It is suitable for laboratory applications requiring reliable protein visualization and analysis.
SERVA Blue G is differentiated from SERVA Blue R by the addition of two methyl groups and a slightly greenish tint to its blue color, resulting in distinct staining characteristics.
The dye exists in multiple forms depending on pH conditions: a cationic (red) form below pH 0 with an absorbance peak at 470 nm, a neutral (green) form around pH 1 with an absorbance peak near 650 nm, and an anionic (blue) form above pH 2 with an absorbance peak at 595 nm.
The blue anionic form binds with amino acid residues, such as arginine and aromatic residues, forming a stable protein-dye complex.
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