SERVA ProteinStain Fluo-R is a highly sensitive fluorescent dye for the detection of proteins in applications such as SDS-PAGE, native PAGE, and 2D gel electrophoresis. It is designed for proteomic workflows requiring reliable and high-contrast protein visualization.
The dye does not interfere with immunodetection, allowing stained gels to be used directly for Western blotting and enabling transfer of protein patterns onto membranes for further analysis.
SERVA ProteinStain Fluo-R can also be used for pre-staining by adding it to the loading buffer instead of conventional tracking dyes such as bromophenol blue. It offers sensitivity comparable to silver staining while providing superior staining properties, making it suitable for advanced proteomic research.
The dye features good linearity and is compatible with MS/MS analysis. It can be combined with silver staining and DIGE techniques for complementary protein detection approaches.
SERVA ProteinStain Fluo-R is easily soluble in water. A 1 µM staining solution can be prepared by dissolving 5 mg in 3 L of water, or a 20 mM stock solution by dissolving 5 mg in 150 µl of water. The dye is optimally excited at 473/488 nm, with 532 nm laser excitation also possible at reduced sensitivity.
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