Highly cross-adsorbed donkey ant-rabbit IgG (H+L) antibody conjugated to VRDye 549.
The antibody was isolated by affinity chromatography using antigens coupled to agarose beads. Based on ELISA, this antibody reacts with heavy and light chains of rabbit IgG and light chains common to most rabbit immunoglobulins. This antibody has been tested by ELISA and/or solid phase adsorbed to ensure minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot applications.
VRDye 549 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C before and after reconstitution.
Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.
| Application | Recommended | Suggested Range |
|---|---|---|
| Western Blot Detection | 1:10,000 | 1:5,000 - 1:15,000 |
| Other | User-optimized |
Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.
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