Western Blot Stripping Buffers
LICORbio Stripping Buffers Help You Conserve Precious Samples and Time
Stripping and reprobing a Western blot is a common laboratory method. Many labs choose to make their own stripping buffer. With pre-made LICORbio Western blot stripping buffers, you can:
- Conserve precious samples by re-using the same blot up to three times to detect different targets or optimize antibody concentrations
- Save time and money
- Effectively remove antibodies, while retaining immobilized proteins
- Strip blots at room temperature in 20 minutes or less without unpleasant odor
- Perform qualitative analysis after stripping
NewBlot™ Stripping Buffers are optimized for stripping and reprobing near-infrared (NIR) fluorescent Western blots, including those detected with IRDye® Secondary Antibodies.
WesternSure® ECL Stripping Buffer is optimized for stripping and reprobing chemiluminescent Western blots.
Which Stripping Buffer Should I Choose?
| Product | Membrane Compatibility | Type of Blot | Performance on Nitrocellulose | Performance on PVDF | Hazardous Shipping |
|---|---|---|---|---|---|
| *WesternSure ECL | Nitro/PVDF | Chemiluminescence |  | | |
| *NewBlot IR | Nitro/PVDF | Near-infrared fluorescence | ** | | |
| NewBlot Nitro | Nitro | Near-infrared fluorescence | | | |
| NewBlot PVDF | PVDF | Near-infrared fluorescence | | |
**Performance is target-dependent. For some targets it works as well as NewBlot Nitro, for others it is not as effective.
