WesternSure PREMIUM Chemiluminescent Substrate is a highly sensitive, enhanced substrate for detecting horseradish peroxidase (HRP) on immunoblots.
Sensitivity comparable with SuperSignal® West Dura for greater than 60 minutes post-incubation
Most intense signal with longest duration
Ideal for use with digital imaging systems
The substrate pack contains Luminol Enhancer Solution and Stable Peroxidase Solution.
Comparison of Three Scans Using WesternSure PREMIUM Substrate
Scan 1Scan 2Scan 3
WesternSure Chemiluminescent Substrate Comparison on C-DiGit® Blot Scanner. WesternSure PREMIUM substrate was used to detect actin in two-fold serial dilutions of C32 lysate (10 μg/well – 1.2 ng/well). Limit of detection was identical (8 bands) after the first two 12-minute scans. WesternSure PREMIUM continued to detect all 8 bands.