2D HPE™™ BlotGels NF Kits are designed for high-resolution separation of proteins by 2D gel electrophoresis followed by efficient blotting for downstream applications, particularly host cell protein (HCP) analysis by 2D Western immunoblotting.
The non-fluorescent (NF) support film also enables excellent results for fluorescent staining and labelling.
These gels are specifically engineered to simplify Western blot transfer from large-format horizontal 2D gels. The gel matrix is covalently bound to the carrier foil along a narrow edge strip, while the remainder of the gel adheres non-covalently. This allows the covalently bound strip to be cut off prior to transfer, enabling easy separation of the gel from the support film and facilitating semi-dry blotting without damaging the gel.
Each kit contains 4 plastic-backed gels, running and equilibration buffers, FS wicks, and cooling contact fluid.
They are suitable for use on the HPE™ BlueHorizon, or Multiphor II™ systems.
Available formats include Triple BlotGel (3 × 7 cm IPG strips), Double BlotGel (2 × 11 cm IPG strips), and Large BlotGel (1 × 24 cm IPG strip), with 12.5% and 10–15% variants depending on format.
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