SERVA PRiME™ Lightning Red Kit is a fluorescent dye kit for rapid pre-labelling of proteins prior to SDS-PAGE and/or Western blotting, eliminating the need for staining and washing steps after electrophoresis.
The labelled proteins enable straightforward normalization in Western blots by using the total protein signal on the membrane as a loading control.
The dye is fully compatible with mass spectrometry and other downstream analytical methods.
Labelling is simple and fast, taking approximately 2–5 minutes for qualitative analysis and around 30 minutes for quantitative analysis. It works with complex and purified samples across a protein concentration range from 1 µg/ml to 20 mg/ml, without requiring protein concentration measurement or purification before or after labelling.
Labelled proteins can be separated using a wide range of gel formats, including precast gels in plastic cassettes such as SERVAGel PRiME™™ Vertical Mini Gels and film-backed gels.
After electrophoresis, detection is performed using a fluorescent imager (camera or scanner) with an excitation wavelength of approximately 530 nm and an emission filter of 610 nm (narrow band width of 30 nm).
Depending on the protocol used, the kit is sufficient for approximately 250–1250 lanes, regardless of gel format (mini, wide, or large).
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